LIF-Free Embryonic Stem Cell Culture in Simulated Microgravity

LIF-Free Embryonic Stem Cell Culture in Simulated Microgravity

BACKGROUND Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required. METHODOLOGY/PRINCIPAL FINDING...

Simulated Microgravity Modulates Differentiation Processes of Embryonic Stem Cells.

BACKGROUND/AIMS Embryonic developmental studies under microgravity conditions in space are very limited. To study the effects of altered gravity on the embryonic development processes we established an in vitro methodology allowing differentiation of mouse embryonic stem cells (mESCs) under simulated microgravity within a fast-rotating clinostat (clinorotation) and capture of microarray-based g...

Effects of Simulated Microgravity on Embryonic Stem Cells

There have been many studies on the biological effects of simulated microgravity (SMG) on differentiated cells or adult stem cells. However, there has been no systematic study on the effects of SMG on embryonic stem (ES) cells. In this study, we investigated various effects (including cell proliferation, cell cycle distribution, cell differentiation, cell adhesion, apoptosis, genomic integrity ...

optimization of buffalo (bubalus bubalis) embryonic stem cell culture system

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P-92: Retinoic Acid and STO Co-Culture System Enhance Mouse Embryonic Stem Cell